NTA is a versatile technique for characterizing nanoparticles in suspension, ranging from 10 to 2000 nm. It enables real-time visualization below the diffraction limit of conventional microscopes, offering applications in extracellular vesicles, lipid bilayer-delimited particles that are naturally released from almost all types of cells. They are implicated in intercellular communication and offer translational value in vaccine development, biomarker discovery and drug delivery.